Sino Biological TNF (TNFA) Recombinant Rat Protein
This recombinant protein was expressed from a DNA sequence encoding the mature form of rat TNF-α (P 16599) (Val 80-Leu 235) fused to an initial Met at the N-terminus
Manufacturer: Invitrogen 80045RNAE5
DescriptionTumor Necrosis Factor (TNF), recombinant rat protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
- Activity: Measured in a cytotoxicity assay using L930 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D; the ED50 is typically 10-20pg/mL
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|TNF (Tumor Necrosis Factor)|
|Tumor necrosis factor|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Tnf, mgC124630, RATTNF, TNF-alpha, Tnfa|
|Cytokines & Receptors|
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