Sino Biological™ TNFRSF1A (CD120a) Recombinant Human Protein, His Tag Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 211) of human TNFRSF1A (NP_001056.1) fused to a polyhistidine tag at the C-terminus.

Manufacturer: Invitrogen 10872H08H50

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Catalog No. 10-872-H08H50

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For Research Use Only. Not for use in diagnostic procedures.


    For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
    Format Lyophilized
    Gene ID (Entrez) 7132
    Molecular Weight 22.7kDa
    Purity ≥90% by SDS-PAGE
    Quantity 50μg
    Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
    Structural Form Truncated/Recombinant
    Protein Subtype TNF (Tumor Necrosis Factor)
    Endotoxin Concentration <1 EU/μg
    Gene Alias CD120a, FPF, mgC19588, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR60, p55, p55-R, p60
    Common Name Tumor necrosis factor receptor superfamily, member 1A
    Gene Symbol TNFRSF1A
    Protein Tag His Tag
    Validated Application Assay Standard
    Protein Family Cytokines & Receptors/CD Proteins
    Expression System Mammalian

    Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A/CD120a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

    • Activity: 1. Measured by its binding ability in a functional ELISA; a. Immobilized human TNFa at 10µg/mL (100µL/well) can bind biotinylated human TNFRSF1A-his, the ED50 of biotinylated human TNFRSF1A-his is 0.04µg/mL; b. Immobilized mouse TNFa at 10µg/mL (100µL/well) can bind biotinylated human TNFRSF1A-his, the ED50 of biotinylated human TNFRSF1A-his is 0.28µg/mL; 2. Measured by its ability to inhibit the TNF mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, the ED50 for this effect is typically 0.04-0.2µg/mL in the presence of 0.25ng/mL recombinant human TNF
    • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
    • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

    Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

  • Description & Specifications

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