Sino Biological™ TNFRSF1A (CD120a) Recombinant Mouse Protein, His Tag Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 212) of mouse TNFRSF1A (NP_ 035739.2) fused to a polyhistidine tag at the C-terminus

Manufacturer: Invitrogen 50496M08H25

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Catalog No. 50-496-M08H25

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For Research Use Only. Not for use in diagnostic procedures.


    For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
    Format Lyophilized
    Gene ID (Entrez) 21937
    Molecular Weight 21.8kDa
    Purity ≥90% by SDS-PAGE
    Quantity 5 x 5μg
    Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
    Structural Form Truncated/Recombinant
    Protein Subtype TNF (Tumor Necrosis Factor)
    Endotoxin Concentration <1 EU/μg
    Gene Alias Tnfrsf1, CD120a, FPF, TNF-R, TNF-R-I, TNF-R1, TNF-R55, TNF-alphaR1, TNFAR, TNFR60, TNFRI
    Common Name Tumor necrosis factor receptor superfamily, member 1A
    Gene Symbol TNFRSF1A
    Protein Tag His Tag
    Validated Application Assay Standard
    Protein Family Cytokines & Receptors/CD Proteins
    Expression System Mammalian

    Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A/CD120a), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

    • Activity: 1. Measured by its binding ability in a functional ELISA: a. Immobilized human TNFA at 10µg/mL (100µL/well) can bind biotinylated mouse TNFRSF1A-his, the ED50 of biotinylated mouse TNFRSF1A-his is 0.28µg/mL; b. Immobilized mouse TNFA at 10µg/mL (100µL/well) can bind biotinylated mouse TNFRSF1A-his, the ED50 of biotinylated mouse TNFRSF1A-his is 0.13µg/mL; 2. Measured by its ability to inhibit the TNF mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, the ED50 for this effect is typically 0.5-1.5µg/mL in the presence of 0.1ng/mL recombinant mouse TNF
    • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
    • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

    Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

  • Description & Specifications

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