SIRP alpha Monoclonal Antibody (15-414), PE, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA174228

Catalog No. PIMA174228

Description

MA1-74228 is specific for human CD172a alpha. Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. Mouse anti Human CD172a antibody, clone 15-414 recognizes human CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells.

CD172a, also known as signal-regulatory protein alpha, is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.

Specifications

• Antigen: SIRP alpha
• Classification: Monoclonal
• Conjugate: PE
• Gene Accession No.: P78324
• Gene Symbols: SIRPA
• Immunogen: Monocyte-derived dendritic cells.
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry
• Clone: 15-414
• Gene: SIRPA
• Gene Alias: BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2, CD172 alpha
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 140885
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Lyophilized