Invitrogen™ SIRT1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5120542

Catalog No. PIPA5120542

Description

Positive test controls include: 293T, HeLa, Jurkat, NIH/3T3, C6. The target is usually found in the following locations: Cytoplasm, Mitochondrion, Nucleus, Nucleus, PML body. Immunogen sequence: VALIPSSIPH EVPQILINRE PLPHLHFDVE LLGDCDVIIN ELCHRLGGEY AKLCCNPVKL SEITEKPPRT QKELAYLSEL PPTPLHVSED SSSPERTSPP DSSVIVTLLD QAAKSNDDLD VSESKGCMEE KPQEVQTSRN VESIAEQMEN PDLKNVGSST GEKNERTSVA GTVRKCWPNR VAKEQISRRL DGNQYLFLPP NRYIFHGAEV YSDSEDDVLS SSSCGSNSDS GTCQSPSLEE PMEDESEIEE FYNGLEDEPD VPERAGGAGF GTDGDDQEAI NEAISVKQEV TDMNYPSNKS.

NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, repsonse to DNA damage, metabolism, apoptosis, and autophagy. SIRT1 can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histone and DNA, leading to transcriptional repression. It is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT).

Specifications

• Antigen: SIRT1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SIRT1
• Gene Alias: 75SirT1; AA673258; hSIR2; hSIRT1; mSIR2a; NAD-dependent deacetylase sirtuin-1; NAD-dependent protein deacetylase sirtuin-1; OTTHUMP00000198111; OTTHUMP00000198112; Regulatory protein SIR2 homolog 1; RP11-57G10.3; SIR2; Sir2a; SIR2alpha; SIR2L1; sir2-like 1; SIR2-like protein 1; SIRT 1; SIRT1; SIRT-1; SirtT1 75 kDa fragment; sirtuin 1; sirtuin 1 ((silent mating type information regulation 2, homolog) 1; sirtuin type 1
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 23411
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry, Western Blot
• Concentration: 2.03 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.3
• Gene Accession No.: Q96EB6
• Gene Symbols: SIRT1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1 (NP_036370.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG