BioVision Inc Sirtuin Activity Assay Kit (Fluorometric): Simple & convenient assay to measure sirt activity in cell & tissue lysate, nuclear extract, mitochondria, purified protein & immunoprecipitated samples. Detection limit: 0.06 µU of recombinant human SIRT6.
Manufacturer: BioVision Inc K324100
Sirtuin Activity Assay Kit (Fluorometric): Sirtuins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins are localized in the cytoplasm, nucleus, nucleolus as well as mitochondria. In BioVision's Sirtuin Activity Assay Kit, the acetylated p53-AFC substrate is deacetylated by Sirtuins in the presence of NAD+ to generate the deacetylated p53-AFC substrate, nicotinamide and O-Acetyl-ADP Ribose. Cleavage of the deacetylated p53-AFC substrate by the Developer releases the fluorescent group, which is detected fluorometrically at Ex/Em = 400/505 nm. HDAC¿s also deacetylate the acetylated p53-AFC substrate. Trichostatin A is added to the reaction to specifically inhibit HDAC¿s in samples. This kit provides a rapid, simple, sensitive, and reliable test to measure Sirtuin Activity in a variety of samples. The limit of quantification of the assay is 0.06 ¿U of recombinant human SIRT6. BioVision charges an Ice pack fee for each order.
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