Invitrogen™ SiteClick™ Antibody Labeling Kits

Generate brightly labeled antibodies for flow cytometry, fluorescence imaging, or western blot detection using SiteClick™ Qdot™ R-PE, and Biotin Antibody Labeling Kits.

Catalog Number	Mfr. No.	Label or Dye	Excitation/Emission	Labeling Scale
S10453	Invitrogen™ S10453	Qdot 655	405/655	100 μg
S10467	Invitrogen™ S10467	R-PE (R-Phycoerythrin)	496, 546, 565/578	100 μg
S10449	Invitrogen™ S10449	Qdot 525	405/525	100 μg
S10454	Invitrogen™ S10454	Qdot 705	405/705	100 μg
S10450A	Invitrogen™ S10450	Qdot 565	405/565	100 μg
S10451	Invitrogen™ S10451	Qdot 585	405/585	100 μg
S10452	Invitrogen™ S10452	Qdot 625	405/625	100 μg
S10455	Invitrogen™ S10455	Qdot 800		100 μg
S10469A	Invitrogen™ S10469	Qdot 605	405/605	100 μg
S20033	Invitrogen™ S20033	Biotin		100 to 250 μg

Description

Generate perfectly labeled antibodies with the SiteClick R-PE, Qdot, or Biotin Antibody Labeling Kits. Site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies, regardless of isotype and host species. Depending upon the label, the resulting SiteClick R-PE, Qdot, or Biotin conjugated antibody can be used in flow cytometry, fluorescence imaging, or Western blot detection. Each kit contains everything needed to label 100 μg of IgG antibody with an easy-to-follow, step-by-step protocol. Achieve highly efficient, site-specific, and reproducible labeling with the SiteClick R-PE, Qdot, or Biotin Antibody Labeling Kits.

Conventional amine or thiol labeling chemistry can result in labeling of the antigen binding domain or compromise the structure of an antibody. SiteClick labeling technology, however, attaches the label under mild conditions to the heavy chains of an IgG antibody, ensuring that the antigen binding domains remain available for binding to your antigen target. Because SiteClick Labeling Kits allow simple and gentle site-selective attachment of detection molecules to heavy chain N-linked glycans — far from the antigen-binding domain — excellent reproducibility is achieved during sequential labelings, as well from antibody to antibody. No harsh reduction steps are required, and antibody labeling is consistent and reproducible each time it is performed.

The SiteClick R-PE, Qdot, or Biotin Antibody labeling workflow consists of three steps:

1. antibody carbohydrate domain modification,
2. azide attachment to the antibody and
3. conjugation with the DIBO-modified label.

This workflow employs copper-free click chemistry to covalently link the label containing the DIBO moiety with the azide-modified antibody. Labeling antibodies with the SiteClick antibody labeling system gives you confidence that the antibody will be labeled the same way every time, with no need for reaction optimization. This means the same number of dyes are attached to each molecule, the antigen binding site is preserved, and the same results are achieved every time.

Important features of the SiteClick Qdot Antibody Labeling Kits:

• Include everything required to label 100 μg of IgG antibody (100–250 μg of IgG antibody with the SiteClick Biotin Antibody Labeling Kit)
• Easy to follow step-by-step protocol that require only a few hours hands-on time to complete.
• Highly efficient, site-specific, reproducible labeling chemistry results in high quality antibody conjugates.

Specifications

• Product Type: Labeling Kit
• Content And Storage: Store the entire kit at 2 to 6°C upon receipt
• Labeling Method: SiteClick Labeling System
• Shipping Condition: Wet Ice