Description: The monoclonal antibody XLP-1D12 reacts with human SLAM-associated protein (SAP), which is also known as SH2D1A. SAP is a 15-kDa SH2 domain-containing adaptor protein. Upon CD150/SLAM family receptor ligation, SAP recruits Fyn tyrosine kinase, which phosphorylates the receptor to activate downstream signaling events. SAP is expressed in thymocytes, T cells, NK cells, and NKT cells. Studies have demonstrated a role for SAP in promoting follicular T helper cell differentiation during germinal center formation. In addition, this protein is involved in NKT cell development, Th1/Th2 differentiation, development of innate T cells, and regulation of NK cell and CD8+ T cell cytotoxicity. Mutations of the gene encoding SAP results in X-linked lymphoproliferative (XLP) syndrome, which is characterized by impaired humoral immunity leading to increased susceptibility to Epstein-Barr virus infection and/or lymphoma. SAP has also been linked to automimmune diseases such as systemic lupus erythematosus. Applications Reported: This XLP-1D12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This XLP-1D12 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transicription Factor Staining Buffer Set (cat. 00-5523) and protocol.Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources tab online. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and maligt lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene.
|SAP (SLAM-Associated Protein)|
|4° C, store in dark, DO NOT FREEZE!|
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
For Research Use Only.
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