SLIT2 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA532586

Catalog No. PIPA532586

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is prostate adenocarcinoma or fetal kidney.

Slit homolog 2 proteins (SLIT2) are ligands for the Roundabout (Robo) receptors and act as molecule guidance cues in cell migration. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. Slit2 protein is expressed in fetal lung and kidney, and adult spinal cord. Weak expression is also noticed in adult adrenal gland, thyroid, and trachea. SLIT2 is a target for repression by EZH2 and is up-regulated in prostate cancer.

Specifications

• Antigen: SLIT2
• Classification: Polyclonal
• Gene: SLIT2
• Gene Alias: SLIL3, Slit-2, slit homolog 2 protein
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9353
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Gene Accession No.: O94813
• Gene Symbols: SLIT2
• Immunogen: Synthetic peptide near C-terminus of human SLIT2
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov