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Smad2/3 Goat anti-Human, Mouse, Polyclonal, R&D Systems™

Goat Polyclonal Antibody

Manufacturer:  R&D Systems AF3797SP

Catalog No. AF3797SP


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Description

Description

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-β) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4); and the inhibitory Smads, (Smads 6 and 7). The binding of TGF-β or activin to their cognate receptor induces phosphorylation of Smads 2 and 3. The activated Smads associate with the common-mediator subunit, Smad4, and the heteromeric complex translocates into the nucleus to initiate transcription. Smad3, also known as Mothers Against Decapentaplegic homolog 3 (MADH3), shares 83% amino acid identity with Smad2, also known as Mothers Against Decapentaplegic homolog 2 (MADH2). Human Smad2 has 99% identity to mouse and rat Smad2. Human Smad3 has 99% identity to mouse and rat Smad3.

Specifications

Specifications

Smad2/3
LYOPH
Western Blot 0.5 ug/mL, Simple Western 25 ug/mL, Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells, Immunocytochemistry 5-15 ug/mL
P84022
hMAD-2, hSMAD2, JV18, JV18-1, JV18-1SMAD, mothers against DPP homolog 2 (Drosophila), MAD homolog 2, Mad protein homolog, Mad, mothers against decapentaplegic homolog 2, MADH2, MADH2MGC34440, MADR2, MADR2MGC22139, Mad-related protein 2, mother against DPP homolog 2, mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD 2, SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila), SMAD, mothers against DPP homolog 2, Smad2, Smox
Goat
IgG
25 ug
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Polyclonal
Detects human, mouse, and rat Smad2/3 in Western blots. Predicted to detect rat based on sequence homology.
Human, Mouse
ChIP assay, Immunocytochemistry, Western Blot
Unconjugated
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Affinity Purified
SMAD2
E. coli-derived recombinant human Smad3 Ser2-Ala230 Accession # P84022
Antigen Affinity-purified
RUO
Primary
4087
Reconstitute at 0.2 mg/mL in sterile PBS.
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