Thermo Scientific SP6 RNA Polymerase: PROMO
Thermo Scientific SP6 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.
Manufacturer: Thermo Scientific EP0133
DescriptionIt catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
- Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)
Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA - T3 TAATACGACTCACTATA G GGAGA - T7 ATTTAGGTGACACTATA G AAGNG - SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).
|SP6 RNA Polymerase|
|Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.|
|1mL of 5X Transcription Buffer|
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