BioVision Inc Sphingomyelin Quantification Colorimetric Assay Kit: Simple & convenient assay to measure sphingomylein in serum, plasma, tissue samples such as brain & adhesion or suspension cells. Detection limit: < 1 µM. 100 assay
Manufacturer: BioVision Inc K600100
Sphingomyelin is an important structural lipid component of cell membranes and lipoproteins. Sphingomyelin has been implicated in the pathogenesis of several diseases; therefore a sensitive and reliable technique for its quantification is of considerable importance. BioVision’s Sphingomyelin Quantification Assay Kit provides a simple and sensitive method for quantifying sphingomyelin using colorimetry. In the assay, sphingomyelin is hydrolyzed into ceramide and phosphorylcholine by sphingomyelinase. Alkaline phosphatase (ALP) dephosphorylates phosphorylcholine to choline, which then reacts with Sphingomyelin Enzyme Mix to produce an intermediate. The intermediate reacts with a highly specific probe to generate color (OD 570 nm). The kit can detect sphingomyelin in various biological samples as low as 1 µM. BioVision charges an Ice pack and Insulated Container fee of $25.00 for each order.
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