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BioVision IncSupplier Diversity Partner Sphingomyelinase Activity Colorimetric Assay Kit: Simple & convenient assay to measure sphingomyleinase activity in tissue samples such as brain & adhesion or suspension cells. Detection limit: < 1.25 mU/ml (~5 µU/mg tissue/cells). 100 assay DFS Item

Manufacturer:  BioVision IncSupplier Diversity Partner K599100

Sphingomyelinase Activity Colorimetric Assay Kit: Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid 2ndmessenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, and cell proliferation. BioVision's SMase Activity Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using colorimetry (OD 570 nm). N-SMase is a Mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce an intermediate that reacts with a highly specific probe to generate color. This assay kit can detect sphingomyelinase activity as low as 1.25 mU/ml (~5 ¿U/mg tissue/cells) in a variety of samples

Catalog No. 50-111-9791

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