SPRTN Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA5107278
Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 92%; Pig: 100%; Rabbit: 92%; Rat: 100%SPRTN is a regulator of UV-induced DNA damage response: acts as a ft.reader ft. of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis. SPRTN is recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. It facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. It is also required for localization of DNA polymerase eta (POLH) to sites of UV damage.
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|PBS with 2% sucrose and 0.09% sodium azide; pH 7.4|
|Synthetic peptide located within the following region: ATNREPSAHD YWWAEHQKTC GGTYIKIKEP ENYSKKGKGK AKLGKEPVLA|