Invitrogen™ SPT5 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA556100

Catalog No. PIPA556100

Description

Immunogen sequence: VGYSPMTPGA PSPGGYNPHT PGSGIEQNSS DWVTTDIQVK VRDTYLDTQV VGQTGVIRSV TGGMCSVYLK DSEKVVSISS EHLEPITPTK NNKVKVILGE DREATGVLLS IDGEDGIVRM DLDEQLKILN LRFL Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 96%.

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. [UniProt].

Specifications

• Antigen: SPT5
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SUPT5H
• Gene Alias: AL033283; AL033321; AU019549; DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; etID10129.15; etID37101.15; fb37g01; fb95e05; fog; foggy; hSPT5; protein foggy; Spt5; SPT5 homolog, DSIF elongation factor subunit; SPT5H; suppressor of Ty 5; suppressor of Ty 5 homolog; suppressor of Ty 5 homolog (S. cerevisiae); Supt5; Supt5h; supt5h protein; Tat-cotransactivator 1 protein; Tat-CT1; Tat-CT1 protein; Transcription elongation factor SPT5; transcription elongation regulator FOGGY; wu:fb37g01; wu:fb95e05; wu:fc10f02
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20924, 308472, 6829
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: O00267, O55201
• Gene Symbols: Supt5, SUPT5H
• Immunogen: Recombinant protein corresponding to Human SPT5. Recombinant protein control fragment (Product #RP-91699).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG