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SSEA3 Rat anti-Human, Mouse, PE, Clone: eBioMC-631 (MC-631), eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$317.00

Specifications

Antigen SSEA3
Clone eBioMC-631 (MC-631)
Target Species Human, Mouse
Conjugate PE
Isotype IgM
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Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-112-20 Non-distribution product as customer accommodation.
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Invitrogen
12883342
100 Tests Each for $317.00
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Description

Description

Description: The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation. Applications Reported: This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioMC-631 (MC-631) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.

SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
Specifications

Specifications

SSEA3
Human, Mouse
IgM
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
eBioMC-631 (MC-631)
PE
Flow Cytometry
Primary
Monoclonal
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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