Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR

SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR).

Supplier:  Invitrogen™ 11752050

Encompass_Preferred

 View more versions of this product

Catalog No. 11-752-050


Add to Cart

Description

Description

SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR). The simple, time-saving reaction set-up uses just two tubes: a 2X reaction mix and an enzyme mix.

Enzyme mix
SuperScript III Reverse Transcriptase, included in the RT enzyme mix, is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 42–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. RNaseOUT Recombinant Ribonuclease Inhibitor, also included in the enzyme mix, is an RNase inhibitor protein that safeguards against the degradation of target RNA due to ribonuclease contamination of the RNA preparation.

Reaction mix
The 2X RT reaction mix includes oligo(dT)20, random hexamers, MgCl2, and dNTPs in a buffer formulation that has been optimized for qRT-PCR. E. coli RNase H is provided as a separate tube in the kit to remove the RNA template from the cDNA:RNA hybrid molecule after first-strand synthesis. This has been shown to increase sensitivity in qRT-PCR.

Using SuperScript III First-Strand Synthesis SuperMix
This SuperMix formulation can be used to quantify fewer than 10 copies of a target gene in qRT-PCR, with a broad dynamic range that supports accurate quantification of high-copy mRNA from up to 1 μg of total RNA. Reagents are provided for 50 or 250 RT reactions of 20 μL each.

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• 2X RT Reaction Mix (500 μL)
• RT Enzyme Mix (100 μL)
E. coli RNase (50 μL)

Store at –20°C.

Super Mix
30 min.
50°C
Dry Ice
First-Strand cDNA
Master Mix
RNA
qPCR
High
Reverse Transcription
SuperScript III
Real Time PCR (qPCR)
50 Reactions
Reverse Transcription
Product Suggestions

Product Suggestions

Safety and Handling

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov
SDS
Documents

Documents

Product Certifications
Promotions

Promotions

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.