|Oligo(dT)20 (50 µM), 50 µL; Random hexamers (50 ng/µL), 250 µL; 5X RT buffer, 1 mL 0.1 M DTT, 250 µL; 10 mM dNTP mix, 250 µL SuperScript® IV RT (10,000 units total at 200 U/µl), 50 µL; RNaseOUT™ (40 U/µL), 100 µL; E. coli RNase H (2 U/µL), 50 µL; DEPC-treated water, 1.2 mL; Total HeLa RNA (10 ng/µL), 20 µL; Sense Control Primer (10 µM), 25 µL; Antisense Control Primer (10 µM), 25 µL|
|All components stored at -5 to -30°C.|
|12.3 kb or less|
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