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Syk Mouse anti-Human, Clone: 4D10.1, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$119.00 - $289.00

Specifications

Antigen Syk
Clone 4D10.1
Target Species Human
Conjugate Unconjugated
Isotype IgG2a, kappa
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-245-584 Non-distribution product as customer accommodation.
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Invitrogen
14669680
25 μg
Each for $119.00
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50-245-585 Non-distribution product as customer accommodation.
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Invitrogen
14669682
100 μg
Each for $289.00
Add to cart
 
Description

Description

Description: This 4D10.1 monoclonal antibody recognizes human Syk, a 72-kDa member of the Syk/ZAP-70 family of non-receptor protein tyrosine kinases. Syk is expressed most highly in B cells, with lower expression in immature thymocytes, mast cells, and platelets. Syk can also be detected in fibroblasts, epithelial cells, hepatocytes, endothelial cells, and neuronal cells. This protein is a major component of signaling cascades downstream of the B and T cell antigen receptors and plays an essential role in lymphocyte development. Upon recruitment to immunoreceptor tyrosine-based activation motifs (ITAMs) on the antigen receptors, Syk is activated by phosphorylation on multiple tyrosines. Once activated, Syk phosphorylates proteins such as phospholipase C gamma and BLNK/SLP-65. Finally, abnormal Syk expression has been linked to tumor cell migration and invasion in several cancers. Applications Reported: This 4D10.1 antibody has been reported for use in flow cytometric analysis and western blotting. Applications Tested: This 4D10.1 antibody has been tested by immunoblotting of reduced Ramos cell lysate. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
Specifications

Specifications

Syk
Human
IgG2a, kappa
Monoclonal
Liquid
Antibody
4° C
4D10.1
Unconjugated
Flow Cytometry, Western Blot
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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