T-bet Mouse anti-Human, Mouse, Rhesus Monkey, eFluor™ 450, Clone: eBio4B10 (4B10), eBioscience™ 

Mouse Monoclonal Antibody

Manufacturer:  eBioscience 48582580

Catalog No. 48-582-580

Description

The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor 450 is an alternative to Pacific Blue.  eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.

Specifications

• Antigen: T-bet
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9JKD8, Q9UL17
• Gene Symbols: TBX21
• Isotype: IgG1, kappa
• Quantity: 25 μg
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!
• Primary or Secondary: Primary
• Gene ID (Entrez): 30009, 57765, 694044

• Applications: Flow Cytometry
• Clone: eBio4B10 (4B10)
• Conjugate: eFluor™ 450
• Gene: TBX21
• Format: Liquid
• Host Species: Mouse
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human, Mouse, Rhesus Monkey