T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
The T4 DNA Ligase requires ATP as a cofactor.
- Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
- Fast - sticky-end ligation is completed in 10 minutes at room temperature
- Supplied with PEG solution for efficient blunt-end ligation
|T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration exceeds 200mM.|
|55.3 kDa monomer|
|E.coli cells with a cloned gene 30 of bacteriophage T4|
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