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Thermo Scientific™ T4 DNA Ligase Promotion

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Manufacturer:  Thermo Scientific™ EL0014

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Catalog No. FEREL0014PM

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

The T4 DNA Ligase requires ATP as a cofactor.

Highlights

  • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
  • Fast - sticky-end ligation is completed in 10 minutes at room temperature
  • Supplied with PEG solution for efficient blunt-end ligation
Specifications

Specifications

5U/μL
E.coli cells with a cloned gene 30 of bacteriophage T4
200U
T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration exceeds 200mM.
55.3 kDa monomer
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