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Applied Biosystems™ TaqMan™ Genotyping Master Mix

Optimized for end-point fluorescence detection in SNP genotyping applications.

Manufacturer:  Applied Biosystems™ 4381657

Encompass

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Catalog No. 43-816-57


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Description

Description

  • Distinct clusters and high call rates for unambiguous allelic discrimination
  • Validated with TaqMan™ SNP Genotyping Assays
  • Excellent pre- and post-PCR stability for high throughput setup and analysis
  • Reaction size is based on a 50μL reaction volume

May be used for a variety of genotyping applications, including:
  • Candidate gene studies
  • Drug-target validation
  • Disease association studies
  • Population genetics
  • Linkage mapping
  • Agricultural applications

Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan™ Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:
  • AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) - Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup
  • Optimized mix components provide excellent specificity for discrimination between alleles
  • Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems™ real-time PCR instruments
  • Single thermal cycling condition for consistent results with TaqMan™ Assays

Validated with TaqMan™ Genotyping Assays and Instruments:
  • TaqMan™ Genotyping Master Mix has been tested across all types of TaqMan™ SNP Genotyping Assays: TaqMan™ Drug Metabolism Genotyping Assays, TaqMan™ SNP Genotyping Assays, and Custom TaqMan™ SNP Genotyping Assays
  • In addition, the master mix is validated with the Applied Biosystems™ thermal cyclers and real-time PCR systems

High-Throughput Setup and Analysis:
  • The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable
  • TaqMan™ Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR
  • This provides the flexibility required to run long experiments, unattended overnight or over a weekend

Reliable Discrimination with Challenging Targets
  • Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions
  • The optimized components of TaqMan™ Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets

Setting the Industry Standard for Quality:
  • Extensive analytical and functional tests are performed for each manufactured lot of TaqMan™ Genotyping Master Mix, including one TaqMan™ SNP Genotyping Assay and one TaqMan™ Drug Metabolism Genotyping Assay
  • Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems™ Web site

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

Specifications

Supplied at 2X concentration. The mix is optimized for SNP genotyping using TaqMan™ probes and contains AmpliTaq Gold™ DNA Polymerase UP (Ultra Pure), dNTPs without dUTP, Passive Reference 1 and optimized mix components. This pack contains two 50 mL bottles, sufficient for 4,000 reactions at the 50 µL total volume.

Storage: 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Primer-Probe
High
AmpliTaq Gold DNA Polymerase
DNA (Genomic)
100 mL
Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known)
TaqMan™
Genotyping Master Mix
2 x 50 mL
2X
2 X
qPCR
Standard
SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation)
Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7
ROX (Pre-mixed)
TaqMan™
UP (Ultra Pure)
Genotyping
SDS
Documents
Product Certifications
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