Applied Biosystems™ TaqMan™ RNA-to-C_T™ 1-Step Kit 

Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.

Catalog Number	Mfr. No.	Sufficient For
NC0855391	Applied Biosystems™ 4392653	40 Reactions
NC0924059	Applied Biosystems™ 4392938	200 Reactions
NC0961600	Applied Biosystems™ 4392656	2000 Reactions

Description

• Accurate across a wide dynamic range for dependable performance
• High sensitivity, enabling low copy number detection and precise quantification
• Validated with TaqMan™ Gene Expression Assays for easy experimental setup
• Consistent performance with a wide variety of targets, including AT-rich, GC-rich and long sequences
• Fewer pipetting steps means less hands-on-time and less chance for errors

Efficiency across a Broad Range of Input Concentrations

• Maximum flexibility in RNA template input quantity requires optimal real-time PCR efficiency across a broad range of template quantities
• The result is comparable to that of the TaqMan™ RNA-to-CT™ 2-Step Kit, and is a significant improvement in dynamic range and sensitivity over the TaqMan™ One-Step RT-PCR Master Mix Reagents.

High Sensitivity at Low Target Concentration

• The sensitivity of TaqMan™ RNA-to-CT™ One-Step Kit was validated using a synthetic RNA template for which copy number is precisely known
• Significant sampling error occurs when measuring low quantities of target, so statistical analysis is required for proper evaluation using multiple replicates
• Statistical analysis indicates high confidence of sample quantification based on a T-test, consistent with as few as 10 copies of target.

Order Info

Shipping Conditions: Wet Ice

Specifications

• Detection Method: Primer-Probe Detection
• Sample Type: RNA

For Research Use Only. Not for use in diagnostic procedures.