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Invitrogen™ TARC/CCL17 Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHCCL17

Encompass

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Catalog No. EHCCL17


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Description

Description

The Human TARC/CCL17 ELISA quantitates Hu TARC in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TARC. Principle of the method The Human TARC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TARC ligand (CCL17) belongs to the CC chemokine family. It is a small cytokine and the gene is located on q arm of chromosome 16, in humans, along with other chemokines. TARC specifically binds to T-cells and interacts with chemokine receptors CCR4 and CCR8. It plays an important role in T cell development in thymus as well as in trafficking and activation of mature T cells.
Specifications

Specifications

96 assays
ELISA Kit
Biotin
Chemokine & Receptors
Q92583
5pg/mL to 2500pg/mL
6361
RUO
2°C to 8°C
4 hr. 45 min.
Immunology
<12%
96 Tests
Plasma, Serum, Supernatant
A-152E5.3,ABCD-2,SCYA17,TARC
Colorimetric Microplate Reader
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Solid-phase sandwich ELISA
HRP
10.2-2500 pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
A-152E5.3, ABCD-2, SCYA17, TARC
<10%
Human
5 pg/mL
SDS
Documents
Product Certifications
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