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TCR β Chain Hamster anti-Mouse, Brilliant Violet 786, Clone: H57-597, BD Optibuild™

Armenian Hamster Monoclonal Antibody

Manufacturer:  BD Biosciences 742484

Catalog No. BDB742484


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Description

Description

The H57-597 antibody reacts with a common epitope of theβchain of the T-cell Receptor (TCR) complex onαβTCR-expressing thymocytes, peripheral T lymphocytes, NK1.1+ thymocytes, and NK-T cells of all mouse strains tested. It does not react withγδTCR-bearing T cells. In the fetal and adult thymus, the TCRβ-chain may form homodimers or pair with the pre-TCRα-chain on the surface of immature thymocytes before TCRα-chain expression. Plate-bound or soluble H57-597 antibody activatesαβTCR-bearing T cells, and plate-bound mAb can induce apoptotic death.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.
Specifications

Specifications

TCR β Chain
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Tcrb, TCRbeta, TCRβ, T cell receptor beta chain
TCR affinity-purified from mouse T-cell hybridoma DO-11.10
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV786 under optimal conditions that minimize unconjugated dye and antibody.
RUO
Primary
Murine
H57-597
Brilliant Violet 786
Affinity Purified
Armenian Hamster
Armenian Hamster IgG2, λ1
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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