TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), PE-Cyanine7, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Supplier:  Invitrogen 25571180

Catalog No. 50-112-3382

Description

Description: The GL3 monoclonal antibody reacts with the gamma delta T-cell Receptor complex (TCR) on all gamma delta TCR bearing T cells, but it does not react with alpha beta TCR. The gamma delta TCR is present on cells in the thymus, epidermis, epithelial lining of the intestine, peritoneal cavity, and lymphoid tissues. Applications Reported: This GL3 (GL-3) antibody has been reported for use in flow cytometric analysis. It is recommended to prestain cells with anti-mouse CD16/32 (cat. 14-0161) to prevent non-specific Fc-mediated binding of the GL3 monoclonal antibody. Applications Tested: This eBioGL3 (GL-3, GL3) antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.

TCR gamma/delta Monoclonal antibody specifically detects TCR gamma/delta in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: TCR gamma/delta
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: 0
• Gene Symbols: Tcrd, Tcrg
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 110066, 110067
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBioGL3 (GL-3, GL3), GL3)
• Conjugate: PE-Cyanine7
• Gene: Tcrd
• Gene Alias: RGD1311300; similar to T cell receptor V delta 6; T cell receptor gamma locus; T3/TCR complex; TCR delta gamma; TCR gamma/delta; TCR-CD3 complex; Tcrg; Trg; Trg@
• Host Species: Armenian Hamster
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

For Research Use Only.