TCR Vβ8 Mouse anti-Human, BUV395, Clone: JR2, BD Optibuild
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 742418
The JR2 monoclonal antibody specifically recognizes the -chain variable region (V 8) of the T-cell antigen receptor expressed on 0.5% to 3% of peripheral blood T cells. This antibody has been further characterized as having specificity to a major subset of the BV8 region as determined in the TCR Workshop of July 1995. Staphylococcal enterotoxin E has been shown to specifically stimulate T cells bearing V 8 (BV8).The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|BV8, TCR Vβ8, TCR V beta 8, TCR Vb8|
|Mouse IgG2b, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Brilliant Ultraviolet 395|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.|
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