TCR V beta F1 Mouse anti-Human, PE-Cyanine7, Clone: 8A3, eBioscience
Mouse Monoclonal Antibody
Manufacturer: LIFE TECHNOLOGIES 25576641
This 8A3 monoclonal antibody recognizes human TCR beta F1, a common framework determit on the beta-subunit of the T cell receptor. Expression of TCR beta F1 has been described as a potential diagnostic marker indicating a favorable outcome for tumor patients with not otherwise specified Nodal Peripheral T-cell Lymphoma (PTCL-NOS). Furthermore, a lack of surface and cytoplasmic TCR beta F1 was also found in small cell variant of T-cell prolymphocytic Leukemia. This antibody will not stain the native form of the TCR and will require fixing and permeabilizing cells. Applications Reported: This 8A3 antibody has been reported for use in flow cytometric analysis, immunofluorescent staining of frozen tissue sections, and immunocytochemistry. Applications Tested: This 8A3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No.00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser The T Cell Receptor alpha beta (TCRab) is a heterodimeric membrane receptor that consists of the highly variable alpha and beta chains expressed as part of a complex with the invariant CD3 proteins. TCRab participates in the activation of T-cells in response to an antigen presented in the context of MHC. Engagement of the TCRab initiates signaling cascades that ultimately result in cellular proliferation, differentiation, cytokine production, and/or activation-induced cell death. These signaling cascades regulate T-cell development, homeostasis, activation, acquisition of effector functions and apoptosis. Cells expressing TCRab constitute the vast majority of T cells in both human and mouse.
|TCR V beta F1|
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Human TCR beta chain constant region|
|4° C, store in dark, DO NOT FREEZE!|
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