TER-119 Monoclonal Antibody (TER-119), eFluor™ 450, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 48592182

Catalog No. 50-163-82

Description

Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin. Applications Reported: This TER-119 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TER-119 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.

TER-119 Monoclonal antibody specifically detects TER-119 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: TER-119
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: 0
• Gene Symbols: Ly76
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 104231
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: TER-119
• Conjugate: eFluor 450
• Gene: Ly76
• Gene Alias: Ly76; Ly-76; TER119
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.