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Invitrogen™ TGFBI (BIGH3) Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHTGFBI

Encompass

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Catalog No. EHTGFBI


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Description

Description

The Human Transforming Growth Factor beta1(TGFβ1) (BIGH3) ELISA quantitates Hu TGFβ1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TGFβ1. Principle of the method The Human TGFβ1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy.
Specifications

Specifications

ELISA Kit
Colorimetric Microplate Reader
Other Proteins
Solid-phase sandwich ELISA
Q15582
25pg/mL to 6000pg/mL
7045
RUO
2°C to 8°C
4 hr. 45 min.
Angiogenesis
<12%
96 Tests
Plasma, Serum, Supernatant
BIGH3,CDB1,CDG2,CDGG1,CSD,CSD1,CSD2,CSD3,EBMD,LCD1
96 assays
This ELISA kit shows no cross-reactivity with the recombinant mouse beta-IGH3.
Biotin
HRP
24.58-6000 pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1
<10%
Human
25 pg/mL
SDS
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Product Certifications
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