Thermo Scientific™ BCIP-T

Enhance blue color development with BCIP-T, a chromogenic substrate for alkaline phosphatase used in conjunction with the oxidant NBT.

Manufacturer:  Thermo Scientific™ R0821

Catalog No. FERR0821

Description

Alkaline phosphatase catalyses the removal of a phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan.

Alkaline phosphatase catalyses the removal of a phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan (1)

Recommended for:

Detection of alkaline phosphatase activity in:

• Southern blotting
• Western blotting
• Northern blotting
• dot/slot blots
• plaque and colony screening

Note:

Preparation of a 50mg/mL stock solution in dimethylformamide is recommended.

Specifications

• For Use With (Application): Detection of alkaline phosphatase activity in:; Southern blotting Western blotting Northern blotting dot/slot blots plaque and colony screening;
• Product Type: BCIP-T
• Quantity: 1g