Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ DyLight™ 550-2xPEG NHS Ester

PEGylated dye for enhanced fluorescence and reduced non-specific binding.

Manufacturer:  Thermo Scientific™ 62269

Catalog No. PI62269


Add to cart

Description

Description

Thermo Scientific DyLight 550-2xPEG Amine-Reactive Dye is a derivative of our high-performance DyLight 550 Dye that can be used to fluorescently label antibodies and other proteins.

The DyLight 550-2xPEG dye contains 2 polyethylene glycol (PEG) chains that are non-cytotoxic, enhance fluorescence and reduce nonspecific binding of conjugates made with them. Conjugates made with DyLight 550-2xPEG Dye can be used as molecular probes for cellular imaging, flow cytometry and other fluorescence detection methods.The PEG chains also improve solubility of the dyes and labeled molecules in aqueous solution, aid in cell permeability and improve tissue retention.

Highlights:

  • High fluorescence intensity - significantly brighter fluorescence than Alexa Fluor™ 555
  • PEGylated - improves solubility in aqueous solution and aids in cell permeability
Applications:
  • Fluorescence microscopy
  • In vivo or ex vivo imaging
  • Cell-based assays
  • Flow cytometry/fluorescence-activated cell sorting (FACS)
Product Details:
DyLight 550-2xPEG Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.
Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to the labeling reagent.

Specifications

Specifications

Amine
562⁄576
DyLight™ 550
Active Ester, Succinimidyl Ester, NHS Ester
Store desiccated at -20°C.
DyLight Fluors
DyLight™
DMF (Dimethylformamide)
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit