Thermo Scientific™ DyLight™ Red Specialty Dyes

Label amine-containing molecules for fluorescence imaging or flow cytometry with these benzopyrillium-based, red-emitting (633 laser) NHS ester fluorescent dyes.

Manufacturer:  Thermo Scientific™ 46633

Catalog No. PI46633

Description

Thermo Scientific™ DyLight Red-emitting Specialty Dyes are optimized for labeling molecules and other staining applications.

DyLight Red-emitting Dyes are a family of labeling agents that provide bright fluorescence detection for imaging. Dyes can be selected based upon their characteristic excitation and emission properties or relative hydrophilicity/hydrophobicity attributes. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetrating ability in vivo, while more hydrophilic dyes have less nonspecific binding potential. Each dye contains an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation.

Highlights:

• Large selection–the largest family of dyes available for red-emitting fluorescence applications

• NHS ester reactive group–allows immediate labeling of antibodies, proteins, peptides, and other amine-containing molecules through amide bond formation

• Multiple solubility options –choose from hydrophilic to hydrophobic dyes to optimize the right dye label for the best performance in a given application

Criteria to consider when choosing a DyLight Red-emitting Specialty Dye:

• Excitation and emission wavelengths–choose the best dye to match the excitation and emission capabilities of your instrument
• Water solubility–choose a dye based on its relative hydrophilicity, which directly correlates to the number of negatively-charged sulfonates it has on its core structure. More hydrophilic dyes are best at maintaining water solubility of a labeled antibody and limiting the nonspecific binding of the conjugate. More hydrophobic dyes often are best at penetrating tissues and cell membranes in vivo, meaning that dyes with fewer sulfonates may work best for some applications.
• DyLight dye selection–the broad selection of red-emitting dyes allows a number of candidate dyes to be tested in a given application for optimal performance

Recommended for:

Fluorescence imaging; Confocal microscopy; Flow cytometry; Spectral fluorescence imaging; In vivo imaging; Fluorescent western blotting; Protein microarrays; Antibody labeling; Peptide labeling; Fluorescence correlation spectroscopy; Protein arrays; Single molecule detection; Nanoparticle conjugation; Biotin/streptavidin conjugation

Specifications

• Description: DyLight 633-B3 NHS Ester
• Formulation: NHS ester activated Benzopyrillium with 3 sulfonate groups
• Sufficient For: 1 use to label a total of 7mg of IgG using typical conditions
• Quantity: 1mg