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Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, High ROX

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Manufacturer:  Thermo Scientific™ K0962

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Catalog No. FERK0962


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Includes:
  • Luminaris HiGreen High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-

Description

Description

  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: ingle copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
Specifications

Specifications

500 x 20μL reactions
Store at -20°C.
Detection Method: SYBR, PCR Method: qPCR
7000 System, 7300 System, 7700 System, 7900HT System, StepOne™ System, StepOnePlus™ System
500 Reactions
DNA, dsDNA
2X
Real time PCR (qPCR), gene expression analysis, siRNA validation, genotyping and pathogen detection
Luminaris HiGreen High ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms and is supplied with nuclease-free water.
Safety and Handling

Safety and Handling

ShelfLife : 2 Years

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