Thermo Scientific™ Luminaris Probe High ROX qPCR Master Mixes

Ready-to-use qPCR master mixes with premixed UDG optimized for probe detection using standard cycling protocol. Master mixes available with or without blue color.

Supplier:  Thermo Scientific™ K0932

Catalog No. FERK0932

Description

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.
The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Excellent qPCR performance
• Specificity - Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity - single copy detection
• Wide linear range - accurate quantification across 9 orders of magnitude
• Reproducibility and convenience - ready-to-use 2X master mix
  • Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

• Luminaris Color HiGreen qPCR Master Mixes include Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; supplied with 40X Yellow Sample Buffer and nuclease-free water.
• Luminaris Color HiGreen High ROX qPCR Master Mix (2X) and Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) contain different amount of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart).
• Luminaris Color HiGreen Fluorescein qPCR Master Mix (2X) contains fluorescein passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart).
• Luminaris HiGreen qPCR Master Mixes include Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized colorless PCR buffer; supplied with nuclease-free water.
• Luminaris HiGreen High ROX qPCR Master Mix (2X) and Luminaris HiGreen Low ROX qPCR Master Mix (2X) contain different amount of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart).
• Luminaris Color HiGreen Fluorescein qPCR Master Mix (2X) contains fluorescein passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart).

Hazardous: No
Shelf Life: 24 Months
Shipping Condition: Frozen Gel Packs
Storage Condition: −20°C
Storage Notes: Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized.
References: M. C. Longo et al., Gene 93, 125-128 (1990).

Specifications

• Content And Storage: 2X Luminaris Probe High ROX qPCR Master Mix (4 x 1.25 mL): includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized colorless PCR buffer; supplied with 40X Yellow Sample Buffer and (4 x 1.25 mL) nuclease-free water. Also contains different amount of ROX passive reference dye for specific real-time platforms. Store the master mix at –20°C for long term storage, or at 4°C for up to one month. ROX dye is sensitive to light and should be protected from direct light. When stored at –20°C, full activity of the mix is retained for at least 24 months as indicated on the tube label.
• Quantity: 500 x 20 μL Reactions
• For Use With (Application): Gene Expression, Genotyping, siRNA Validation, Pathogen Detection
• Concentration: 2X

Safety and Handling

ShelfLife : 2 Years