Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)

PCR directly from various animal tissues including mice, fish, birds and insects without DNA purification.

Supplier:  Thermo Scientific™ F140WH

Catalog No. F140WH

Description

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized reagents, sampling tools, and DNARelease Additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes detailed instructions and control primers that can be used with numerous animal species.
Animal Direct PCR Protocol
Highlights
• No need for time-consuming and expensive DNA purification steps
• Very little sample material required
• Two simple protocols for various applications
• Convenient sampling tools included in the kit
•Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb).
Includes
• Phire Hot Start II DNA Polymerase
•Reaction voume with dilution & storage protocol: 20 μL
•Reaction voume with direct protocol: 50 μL
• 2X Phire Animal Tissue PCR Buffer (includes dNTPs and MgCl₂)
• Universal control primer mix
• Dilution Buffer
• DNARelease Additive
• Gel loading dye
Two alternative protocols suitable for various purposes
In the direct protocol, a small piece of animal tissue is placed directly into the PCR reaction. In the dilution protocol, a small amount of animal tissue is first briefly incubated in incubation buffer and 1 μL of this buffer is then added into the PCR reaction. The dilution protocol is useful if several PCR reactions are performed from the same sample.

Specifications

• Content And Storage: Contains:
  • 200 μL Phire Hot Start II DNA Polymerase
  • 5 x 1.25 mL 2X Phire Animal Tissue PCR Buffer (includes dNTPs and MgCl2)
  • 40 μL Control Primer mix (25 μM each)
  • 2 x 5 mL Dilution Buffer
  • 3 x 100 μL DNARelease Additive
  • 3 x 1 mL Gel loading dye
  
  Store at -20°C.
• PCR Method: Direct PCR
• Reaction Speed: Fast
• Product Type: Direct PCR Kit
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Sample Type: Tissue

• GC-Rich PCR Performance: Medium
• Polymerase: Phire Hot Start II DNA polymerase
• Product Line: Phire
• Sufficient For: 500 x 20 μL Reactions
• No. of Reactions: 500 x 20 μL Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.