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Thermo Scientific™ Phusion Green GC Buffer Pack

Buffer packs for use in PCR with Phusion High-Fidelity DNA Polymerases

Manufacturer:  Thermo Scientific™ F539L

Catalog No. F539L


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Includes: Phusion GC Buffer Pack
  • 5X Phusion GC Buffer (1X buffer provides 1.5 mM MgCl2)
  • 50 mM MgCl2
  • DMSO
Phusion Green GC Buffer Pack
  • 5X Phusion Green GC Buffer (1X buffer provides 1.5 mM MgCl2)
  • 50 mM MgCl2
  • DMSO

Description

Description

GC Buffer for Phusion DNA Polymerases

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases: 5X Phusion HF Buffer and 5X Phusion GC Buffer. The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases: 5X Phusion HF Buffer and 5X Phusion GC Buffer. The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.
5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Includes
Phusion HF Buffer Pack (F-518):

  • 5X Phusion HF Buffer (1X buffer provides 1.5 mM MgCl2)
  • 50 mM MgCl2
  • DMSO
Phusion GC Buffer Pack (F-519):
  • 5X Phusion GC Buffer (1X buffer provides 1.5 mM MgCl2)
  • 50 mM MgCl2
  • DMSO
Phusion Green HF Buffer Pack (F-538L):
  • 5X Phusion Green HF Buffer (1X buffer provides 1.5 mM MgCl2)
  • 50 mM MgCl2
  • DMSO
Phusion Green GC Buffer Pack (F-539L):
  • 5X Phusion Green GC Buffer (1X buffer provides 1.5 mM MgCl2)
  • 50 mM MgCl2
  • DMSO
Phusion HF Buffer Pack, Detergent-free (F-520):
  • Detergent-free 5X Phusion HF Buffer (1X buffer provides 1.5 mM MgCl2)
  • 50 mM MgCl2
  • DMSO
Phusion GC Buffer Pack, Detergent-free (F-521):
  • Detergent-free 5X Phusion GC Buffer (1X buffer provides 1.5 mM MgCl2)
  • 50 mM MgCl2
  • DMSO

Specifications

Specifications

Phusion Green GC Buffer Pack (F-539L) includes:
• 5X Phusion Green GC Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

Store at -20°C.
Phusion High-Fidelity DNA Polymerase
4 x 1.5 mL
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