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Thermo Scientific™ Pierce™ Color Silver Stain Kit

Detect proteins with this metallic silver staining technique that produces color variations to differentiate protein bands and spots in 1D and 2D gels.

Manufacturer:  Thermo Scientific™ 24597

Catalog No. PI24597

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Thermo Scientific™ Pierce Color Silver Stain Kit enables sensitive detection of proteins electrophoresed in 1D and 2D polyacrylamide gels using a metallic silver technique which produces color variations that facilitate differentiation of protein bands and spots.

The Color Silver Stain Kit contains four reagents and a flexible protocol for staining proteins in 1D and 2D electrophoresis gels. The method requires more time than monochromatic silver stains but provides greater flexibility in adapting the concentration of working solutions to obtain effective staining with different gel types and thicknesses and unusual protein targets. Proteins stain as black, blue, brown, red or yellow bands or spots according to a combination of their specific charge and other characteristics. Once results are known for a particular protein of interest, these slight color differences provide for easy band and spot identification.


  • Customizable – kit is a reagent set that allows customized formulation of working reagents and staining protocols for particular gel formats and silver-staining applications

  • Sensitive – detects many proteins at 0.1ng per square millimeter in standard-thickness gels

  • Broad protein detectability – stains a wider range of protein types than monochromatic silver stains (proteins that do not bind silver are revealed as yellow bands or spots)

  • Color differentiation – gel-separated proteins and polypeptide-containing macromolecules stain in five basic colors: black, blue, brown, red and yellow
  • Profiling resolution – multicolored detection of DNA, lipids and polysaccharides in addition to typical proteins provides additional landmarks to aid in 2D gel profiling


Four reagent solutions (500mL each): silver, reducer base, reducer aldehyde and stabilizer base



In-Gel Detection
Kit contains sufficient reagents to stain 25 (18 cm2) or 40 (130 cm2) gels
• 500 mL Silver Reagent
• 500 mL Reducer Aldehyde Reagent
• 500 mL Reducer Base Reagent
• 500 mL Stabilizer Base Reagent
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