Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit
Simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.
Manufacturer: Thermo Scientific 16183
The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.
The Pierce Cypridina-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Cypridina and Red Firefly luciferase activity. Cypridina luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. This reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.
- Simultaneous concurrent, filter-based, wavelength-separated detection of two luciferase activities
- Sensitive measure bright blue Cypridina and red firefly luciferase activities in the same sample
- Fast no quenching step required, unlike in traditional sequential dual assays
- Multiplex capable of quantitating two cellular activities in the same sample(s)
Cell lysis buffer, reaction buffer, and substrates Vargulin and D-luciferin
Cypridina and Red Firefly luciferase reporters; Filter sets in the ranges of 425 to 525nm for Cypridina Luc and 615 to 675nm for Red Firefly Luc. Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time.
Study two regulatory elements at the same time; Monitor two signaling pathways simultaneously; Enable studying more than one target per screen (e.g. off-target effects)
|Reporter Enzyme, Luciferase Reporter Assay|
|Sufficient For: 100 dual-luciferase assays in microplate wells
• Cypridina-Firefly Dual Assay Buffer, 5 mL (store at 4°C)
• 100X Vargulin, 50 µL (store at -20°C)
• D-Luciferin, 3 mg (store at 4°C)
• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)
Upon receipt, store kit at -20°C, or store individual components as indicated above.
|Vargulin (Cypridina Luciferin), D-Luciferin|
|384-well plate, 96-well plate|
|Luciferase, Cypridina Luciferase|
|100 rxn kit|
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