Thermo Scientific™ Pierce™ STAT6 In-Cell ELISA Kit

Measure total and phosphorylated signal transducers and activators of transcription 6 using antibodies detected by fluorescence or TMB substrate in whole-cell plate assays.

Manufacturer:  Thermo Scientific™ 62208

Catalog No. PI62208

Description

Thermo Scientific™ Pierce STAT6 In-Cell ELISA Kits are colorimetric and fluorescent microplate assays optimized to measure total and phosphorylated signal transducers and activators of transciption 6 (phospho-STAT6) in whole cells.

Pierce STAT6 In-Cell ELISA Kits enable the relative quantitation of total and phosphorylated signal transducers and activators of transciption 6 (STAT6) in whole cells using a 96- or 384-well microplate format. The complete kits include a mouse monoclonal anti-STAT6 antibody, rabbit monoclonal anti-phospho-(Tyr641) STAT6 antibody, detection reagents and all buffers required for either colorimetric or near infrared detection.

The Colorimetric In-cell ELISA Kit facilitates comparison of multiple target protein levels from different wells using target-specific antibodies, HRP-conjugated detection reagents and TMB substrate. Results across wells are then normalized to cell number based on whole-cell staining with Janus Green.

The Near Infrared In-Cell ELISA Kit allows simultaneous detection of two different target proteins within the same well, eliminating variability caused by differences in cell plating. With the near IR system, protein levels are determined by using target-specific primary antibodies and DyLight™ Fluor-Conjugated Secondary Antibodies. The near IR detection method eliminates issues with background fluorescence, providing a high signal-to-noise ratio and making these fluorescent multiplex experiments both sensitive and robust.

Highlights:

• In-cell ELISA – target-specific detection directly from plated whole cells; no cell lysis, protein extraction or special sample preparation required

• Quantitative and specific – system provides accurate measurement of relative protein expression levels in treated vs. nontreated cells

• Better than Western blotting – In-cell ELISA directly yields quantitative results and provides higher throughput than Western blotting techniques

• Less expensive – costs less (one well vs. one lane) than Western blotting methods of protein expression and activation analysis

• Multiplex-capable – replicate wells or different fluors enable multiple ELISA targets to be measured relative to the primary targets supplied with the kit

• Two kit formats – choose the colorimetric kit for use with standard microplate readers; choose the near infrared fluorescent detection kit for use with IR imaging systems

Includes:

Anti-Phospho STAT6 (Y641) Antibody, Anti-STAT6 Antibody, secondary antibodies, buffers, detergents and plate seals

Specifications

• Description: Pierce STAT6 Colorimetric In-Cell ELISA Kit
• Kit Contents: 20X Tris Buffered Saline, 50mL
• Quantity: 96 Test Kit

• Format: Multi-component kit with HRP-conjugate
• Sufficient For: 96 microplate wells of overnight-cultured cells