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Thermo Scientific™ ThermoPrme™ Alternatve MgClsub2sub Concentraton Taq DNA Polymerase

For PCR applications requiring increased yield and sensitivity, these polymerases are optimized to produce large amounts of PCR product.

Specifications

For Use With (Application) Routine PCR using genomic, viral and plasmid templates; Amplifying fragments up to 12kb; RT-PCR
Includes 0.025U/µL ThermoPrime Plus DNA Polymerase (=1.25U/50µL rxn); 75mM Tris-HCl (pH 8.8 at 25°C); 20mM (NH4)2SO4; 0.01% (v/v) Tween™; 200µM of each dATP, dCTP, dGTP, dTTP; 1.5-4.0mM MgCl2 (depending on Cat. No. selected)
Storage Requirements Store at -20°C for long-term storage. Stable at 4°C for up to four weeks. Avoid freeze-thaw cycles.
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Catalog Number Mfr. No. Description Concentration Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Description Concentration Quantity Price Quantity & Availability  
AB-0575DCB
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Thermo Scientific™
AB0575DCB
ThermoPrime DNA Polymerase Master Mix 2X 1600 x 25uL rxn This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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AB-0575LDB
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Thermo Scientific™
AB0575LDB
ThermoPrime DNA Polymerase ReddyMix Master Mix 1.1X 1600 x 25uL rxn This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

These Thermo Scientific™ ThermoPrime Taq DNA Polymerases are recommended for PCR applications requiring increased yield and sensitivity and to produce large amounts of PCR product.

Thermo Scientific ThermoPrime Taq DNA Polymerase is a robust enzyme that delivers high reaction sensitivity and reliable PCR results. Optimized buffers work synergistically with the ThermoPrime enzyme to produce high yield, full-length DNA, up to 12kb in size.
  • High Purity—94kDa ultrapure recombinant thermostable DNA polymerase provides 5' to 3' exonuclease (strand displacement) activity and 3' adenylation activity

  • High thermal stability—Proprietary buffer formulation protects ThermoPrime Taq during high temperature steps, ensuring optimal performance throughout the PCR reaction

  • Full-length amplification—ThermoPrime Taq is proven to amplify standard DNA targets in excess of 12kb

  • Simple protocol—Master mix formats reduce protocol steps and minimize the risk of pipetting errors and sample contamination and are available in ReddyMix format for direct loading onto agarose gels

  • Reproducible results—ThermoPrime Taq is fully licensed for PCR, and all mixes undergo extensive quality control testing to ensure batch-to-batch consistency

  • Mix contains: 0.025U/µL ThermoPrime Plus DNA Polymerase (=1.25U/50µL rxn); 75mM Tris-HCl (pH 8.8 at 25°C); 20mM (NH4)2SO4; 0.01% (v/v) Tween™; 200µM of each dATP, dCTP, dGTP, dTTP; 1.5-4.0mM MgCl2 (depending on Cat. No. selected)

Recommended for:

Routine PCR using genomic, viral and plasmid templates; Amplifying fragments up to 12kb; RT-PCR

Specifications

Specifications

Routine PCR using genomic, viral and plasmid templates; Amplifying fragments up to 12kb; RT-PCR
0.025U/µL ThermoPrime Plus DNA Polymerase (=1.25U/50µL rxn); 75mM Tris-HCl (pH 8.8 at 25°C); 20mM (NH4)2SO4; 0.01% (v/v) Tween™; 200µM of each dATP, dCTP, dGTP, dTTP; 1.5-4.0mM MgCl2 (depending on Cat. No. selected)
Store at -20°C for long-term storage. Stable at 4°C for up to four weeks. Avoid freeze-thaw cycles.
SDS

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

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