Molecular Probes™ ThiolTracker™ Violet (glutathione detection reagent)

ThiolTracker™ Violet (glutathione detection reagent)

Supplier:  Molecular Probes™ T10095

Catalog No. T10095

Description

ThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of intracellular free thiols in the cell, ThiolTracker™ Violet can be used in estimating the cellular level of reduced glutathione.
ThiolTracker™ Violet offers the following;

10X brighter than mBCL

UV or 405 nm excitation, 525 nm emission

Easy to use

Reproducible results

Can be fixed with aldehydes and permeabilized by Triton™ X-100 (0.5%)

Formaldehyde fixable and suvives detergent extraction

Can be used in multiplex assays including cytotoxicity studies
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection. ThiolTracker™ Violet can be used for Flow Cytometry, HCS imaging, and epifluorescent microscopy.

Specifications

• Color: Violet
• Excitation Wavelength Range: 405⁄526
• Format: Tube(s), Slide(s)
• Quantity: 180 rxn(s)
• Emission: Visible
• Product Line: Molecular Probes™, ThiolTracker™
• Label Type: Other Labels or Dyes

• Content And Storage:

  Store at ≤–20°C, L protect material from long-term exposure to light; may be exposed to light for short periods of time, requires DMSO

• Dye Type: ThiolTracker™ Violet
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
• Detection Method: Fluorescent
• Form: Lyophilized
• Shipping Condition: Room Temperature
• Product Type: Actin

For Research Use Only. Not for use in diagnostic procedures.