Description: This 2POK monoclonal antibody reacts with mouse ThPOK, which is also known as c-Krox and Zbtb7b. In hematopoietic cells, this 78 kDa transcription factor is expressed in thymocytes and T lymphocytes in the spleen and lymph nodes. In non-hematopoietic cells, ThPOK can be detected in the skin, heart, smooth muscle, and liver. Studies demonstrate that ThPOK promotes CD4+ lineage differentiation by inhibiting the expression of CD8 lineage-specific genes such as Runx3. ThPOK has also been shown to inhibit Cd4 silencing, another key facet required for differentiation to the CD4+ lineage. In addition to its role in thymocyte development, ThPOK is involved in the effector functions of invariant natural killer T (iNKT) cells and gamma delta T cells. Applications Reported: This 2POK antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 2POK antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. ZFP67 is an early growth response gene that encodes a zinc finger-containing transcription factor that binds to the promoter regions of type I collagen genes (e.g., COL1A1; MIM 120150) and has a role in development.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.