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Invitrogen™ TNF alpha Bovine ELISA Kit
GSA_VA

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EBTNF

Encompass

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Catalog No. EBTNF


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Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

Description

The Bovine Tumor Necrosis Factor alpha (Bov TNFα) ELISA quantitates Bov TNFα in bovine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Bov TNFα. Principle of the method The Bovine TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Tumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF-alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF-alpha. Due to its varied actions throughout the immune system, TNF-alpha may play a role in the pathogenesis of many disease states. TNF-alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF-alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF-alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF-alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.

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Specifications

Specifications

This ELISA antibody pair detects bovine TNF-alpha. Other species not determined
Colorimetric Microplate Reader
Solid-phase sandwich ELISA
Kinase & Inhibitors
Q06599
0.1 ng/ml to 30 ng/ml
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
5 hr.
RUO
2°C to 8°C
4 hr. 45 min.
TNF alpha
BOS_21587-a, TNF-alpha, TNFa, TNF
<10%
Bovine
0.1 ng/mL
Colorimetric
ELISA Kit
96 Assays
Biotin
HRP
0.123-30 ng/mL
TNF-a (TNF) ELISA Kit, 96 Assays, 0.1 to 30ng/mL Test Range, 5 hr. Test Time, 0.1ng/mL Sensitivity, Cell Culture Supernatants, Plasma, Serum Sample, Cattle/Bovine Species, Colorimetric Detection Method, TNF Gene, Used for Protein Assays and Analysis, 2 to
280943
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Pre-coated 96-well plate, standard, detector antibody, streptavidin-HRP, diluents, wash buffer, chromogen, stop solution and plate covers
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
1 hr. 20 min.
Bovine
Extracellular Matrix & Cell Adhesion
<12%
96 Tests
Plasma, Serum, Supernatant
TNF-a,TNF-alpha,TNFa
SDS
Documents
Product Certifications
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