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TNF alpha Monoclonal Antibody (MP6-XT22), APC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Supplier: Invitrogen 17732181
Description
Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane-bound form. Applications Reported: The MP6-XT22 antibody has been reported for use intracellular staining for flow cytometric analysis. Applications Tested: The MP6-XT22 antibody has been tested by intracellular staining with flow cytometric analysis. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.
TNF alpha Monoclonal antibody specifically detects TNF alpha in Mouse samples. It is validated for Flow Cytometry.Specifications
TNF alpha | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P06804 | |
Tnf | |
Affinity chromatography | |
RUO | |
21926 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
MP6-XT22 | |
APC | |
Tnf | |
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor | |
Rat | |
50 μg | |
Primary | |
Mouse | |
Antibody | |
IgG1 κ |
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For Research Use Only.