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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells

Manufacturer:  Invitrogen™ 452640


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Catalog No. 45-264-0

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Includes: Topoisomerase I-activated pCR™II-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water



Includes the pCR™II-TOPO TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable cloning method, featuring a 5-minute cloning reaction and 3-step protocol, and yielding up to 95% recombinants. Resistant to Kanamycin (KanR) andZeocin™ (ZeoR)
Kit Options: TOPO TA Cloning Dual Promoter Kits
TOPO TA Cloning Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs:

  • General cloning: TOP10 cells (Cat. Nos. K4600-J10, K4600-01, K4600-40)
  • High-efficiency cloning: TOP10 Electrocomp™ cells (Cat. Nos. K4660-01, K4660-40)
  • General cloning, bacteriophage T1 resistance: DH5α-T1R cells (Cat. Nos. K4620-01, K4620-40)
  • Fast growth: Mach1™ -T1R chemically competent E. coli (Cat. No. K4610-20)
  • Repressor/induction needs: TOP10F' cells (Cat. Nos. K4650-01, K4650-40)
  • Provide your own cells (Cat. Nos. 450640 and 452640)

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and SP6 promoters for efficient in vitro transcription
  • M13 forward and reverse primer sites for sequencing
  • 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
  • Kanamycin and ampicillin resistance for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants

Cloning, PCR Cloning



Cloning and subsequent in vitro transcription
TOPO™ TA Cloning™ Kit
Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water
10 Reactions
T7, SP6
Ampicillin (AmpR), Kanamycin (KanR)
TOPO-TA Vectors
Store at -5° to -30°C.
Safety and Handling

Safety and Handling

ShelfLife : All reagents are stable for 6 months when properly stored.

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