DescriptionToxBLAzer DualScreen Kit provide the key advantage of combining the ratiometric reporter gene readout of beta-lactamase with a cytotoxicity readout in the same assay. Thus, ToxBLAzer DualScreen yields reporter readouts that normalize cytotoxic cellular responses, which is a necessity in screening applications which down regulate transcription. The ToxBLAzer substrate contains both the LiveBLAzer-FRET B/G substrate and a proprietary cytotoxicity indicator. Both compounds readily enter the cell, where endogenous esterases rapidly convert them into fluorescent compounds Esterase activity and an intact cell membrane are required for detection of the indicator. The cytotoxicity indicator's fluorescence is red-shifted to ensure compatibility with the blue and green emissions of the LiveBLAzer-FRET B/G substrate, allowing detection of both reporter gene activity and cytotoxicity in the same assay sample.
Advantages of ToxBLAzer DualScreen Kits:
|ToxBLAzer™ DualScreen Kit|
For Research Use Only. Not for use in diagnostic procedures.
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