TRA-1-60 (Podocalyxin) Mouse anti-Human, PE, Clone: TRA-1-60, eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 12886380
Description: The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer. Applications Reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Keratan sulfate antigen TRA1-60; TRA160|
|4° C, store in dark, DO NOT FREEZE!|
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