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TRA-1-60 (Podocalyxin) Mouse anti-Human, PerCP-eFluor™ 710, Clone: TRA-1-60, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 46886382

Catalog No. 50-112-3920


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Description

Description

Description: The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer. Applications Reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the 2102Ep cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
Specifications

Specifications

TRA-1-60 (podocalyxin)
Monoclonal
0.2 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Keratan sulfate antigen TRA1-60; TRA160
IgM
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
Human
Flow Cytometry
TRA-1-60
PerCP-eFluor™ 710
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
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